FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2140629
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGAC1459.180894E-734.762433
GTATTAA4400.034.36741
TCTGTCG7500.033.5989728
GTCTCGC7500.032.6435781
GTCTTGC18950.029.2591421
GTATTAT4450.029.1267851
TCGCTCT9600.027.7523924
GATAACG1607.155901E-527.0029562
TCTCGCT9900.026.9120372
CTCGCTC10450.026.1846873
GTGTTAG13300.025.4463961
GTGTAAA11000.024.8754521
CTGTCGC11400.024.6308259
TTGCTCT22650.023.5252064
GAGTGCA53100.022.915626
GTTCTAC5350.022.8810121
GGAGTGC47600.022.6894385
TCTCGAT7300.022.6842377
GTTCTAT7100.022.3123041
TCTTCGC2303.317473E-521.9123738
Produced by FastQC (version 0.11.5)