FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2189747
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8850.034.1719671
GTCTTGC18900.032.764281
TCTGTCG8250.030.5441828
GTGTTAC8750.028.8020881
TCGCTCT10150.028.3751454
CGGTTAC1050.00797240727.430561
GTATTAA5850.027.0788861
GGTATAC3850.026.1837161
TCTCGCT11350.026.0106952
CTCGCTC10500.025.3732683
GTGTAAC6500.024.3709961
GTCTTAC7450.024.1628251
CGCTCTG9850.023.3898875
ACCGTGT6500.023.2605678
TTCACCG6850.023.1231235
TTGCTCT23100.023.0655544
TCTGTCA25800.022.6037088
GTGTATA8650.022.4756181
GGAGTGC49150.022.1191185
GTATAAG6200.022.0661161
Produced by FastQC (version 0.11.5)