FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2189747
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9150.040.129338
GTCTCGC9050.035.0055351
GTATAAG5950.033.8823621
GTCTTGC18650.031.2707881
CGTTTCG1204.2286917E-429.998649
TCGCTCT11500.028.1726364
CTGTCGC13600.027.5281649
CTCGCTC12500.026.4941943
GTATTAC4500.025.6000081
GTCTTAC7050.025.5319211
CGCTCTG11600.025.4471235
GTCCTAC6050.024.9917431
GTCCTAT4700.024.5106451
TCTCGCT12800.024.185852
GTATTAG7200.024.000011
GTATACG2101.6418195E-524.0000081
TATTACG1500.00155692423.9983632
TTGCTCT24050.023.6496544
GTCTCAC22700.023.4713731
TAGGGCG2151.9704286E-523.4407964
Produced by FastQC (version 0.11.5)