FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2133851
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9550.042.219138
GTCTTGC18350.035.3127141
GTCTCGC10150.031.9206091
GTATTAA4750.030.3152431
CTGTCGC13150.029.0184889
TTATCCG1306.7538617E-427.6918074
TCGCTCT10400.027.6918074
GTATTAT6450.026.7902131
TACCCGT1907.5568805E-626.5258355
GTACTAC3555.456968E-1226.365721
TACACTA4400.026.1813475
CGCTCTG11450.025.7811935
GTATAAT6250.025.3435441
TCTCGCT12350.025.0683732
TTGCTCT25050.024.7181174
TATAGTA4850.023.752152
CTTGCTC24600.023.4142113
GTACTAG3702.8376235E-1023.350931
GTCCCGT2502.4230085E-623.0395836
GTAATAC4403.6379788E-1222.9086783
Produced by FastQC (version 0.11.5)