FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1987723
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAC803.8909657E-545.0017051
TCTGTCG7250.041.7087758
GTCTTGC17550.031.5909391
TCGCTCT9600.031.5011944
GTCTTAC6700.031.1653611
GTCTCGC9300.029.420471
GTACTAT3550.028.3954431
GTATTAA4850.028.2072541
CTGTCGC10850.027.869929
ATAATAC4850.026.7226623
GTGTAAC6100.025.9681991
GTCTCAC18650.025.4808581
GTGTACG2601.2248347E-724.924021
TAATACT4500.024.0009124
GTACTAA3052.1917003E-823.6074521
GGAGTGC42750.023.4102365
CGCTCTG10550.023.2035055
CTCGCTC12450.023.1334083
TCTCGCT12150.022.5193732
GAGTGCA48150.021.6819736
Produced by FastQC (version 0.11.5)