FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2007293
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAC803.8936236E-544.996533
TCTGTCG9450.031.9999248
GTCTCGC7900.031.8962751
TAGTACT3300.030.5453824
TCGCTCT8850.030.1016244
GTATTAA6050.029.749771
GTCTTGC16800.029.140611
GTGTTAC7300.027.6143071
GTTAGCC9850.027.0435963
TTAGACG1400.001041021525.7142244
GTGTTAG13000.024.367351
GTCTTAC7700.024.3098141
TGTTAGC11050.024.1067372
CGCTCTG10450.023.4257815
GTCTCAC19700.023.3890571
CTGTCGC13050.023.1723589
GTATAGG5350.022.876741
GTACTGG9250.022.571231
GTATAAG5500.022.252831
GGTCTAC4557.2759576E-1222.1521381
Produced by FastQC (version 0.11.5)