FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2555134
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10150.048.988641
TCGCTCT12000.037.232554
TCTCGCT12800.036.594492
TCTGTCG9750.033.229268
CTCGCTC13700.032.0864453
CGCTCTG13150.031.2077055
CTGTCGC12400.028.450329
GTCTTGC21950.025.607811
GTATTAT8350.024.1648391
GTATTAC5750.023.8121221
GTGCTAA9350.023.1218191
GTTTCAC37400.022.7364543
GTGTATA11850.022.5006831
GTCCTAC6100.022.4458521
GTCTAAC5200.022.1732311
TTATACT8250.021.8372754
GAGTGCA61500.021.5404826
GTCTCAC28500.021.239621
GGAGTGC56350.020.9537945
TAATACT7950.020.8484154
Produced by FastQC (version 0.11.5)