FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2127474
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7700.043.010068
GTCTCGC9300.035.6523741
CTGTCGC10200.032.468389
GTCTTGC17600.031.5349141
TCGCTCT9500.030.3479614
CGAACTA1000.006286985728.798049
GGTATAC2904.2382453E-1027.3406141
GTCTCAC21600.026.6962221
TCTCGCT10650.026.3941782
GTCCTAC5900.025.6555211
GTACAGA10200.024.7332671
GTGTTAG14700.024.516941
GTATAGA7650.024.4977111
GTATTAG6850.024.2019771
CTCGCTC11050.024.1341843
GTATTGA6900.024.0266021
GTACCCG1500.001546285924.02661
GTTAGCC9600.024.0254693
CGCTCTG10800.023.9994955
GTGTAAG9400.023.7709981
Produced by FastQC (version 0.11.5)