FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2522734
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10200.043.7976231
TCTGTCG9550.042.218158
TCTCGCT12250.034.703632
TCGCTCT12600.033.7396434
CTCGCTC13000.030.4844513
CTGTCGC12700.030.0460059
CGCTCTG13950.029.418145
GCGTAAT1400.001036524725.7336251
TAATACT7200.025.0188034
GTCTTAC9700.024.5132681
GTCTTGC22000.023.5813581
GTATTAC6500.023.2790361
GTTTCAC38000.022.9435583
GTATAAT6700.022.5841371
TAGGGCG2252.7831109E-522.416854
GCGTTAA1650.002696811521.834591
GGTCTAT7800.021.2467371
GTATTAG9600.021.0157951
GTCTCAC28200.020.6964051
GTGTTAC10150.020.58691
Produced by FastQC (version 0.11.5)