FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2522734
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11500.041.3214158
CTGTCGC15200.034.578689
CGTACAC2501.8189894E-1234.5590443
TCGCTCT11600.034.136994
GTCTCGC10050.031.5308881
GTCTTGC24550.030.2158871
CGCTCTG12800.028.6867075
TCTCGCT12750.025.4150922
GTATTAA7250.024.8342841
CTCGCTC13350.024.8083043
GTACCGT1751.3144973E-424.6923731
GTACGGA2101.6385276E-524.0064721
GTGCTAC8900.022.6577931
CGTTTCG1600.002265457322.4998259
GTCTTAC8800.022.0968671
GTATAGG7500.022.0859571
TACACTA5950.021.780915
TTGCTCT31150.021.7265264
CGTACTG2353.9163962E-521.4462155
GTATAAA9750.021.421161
Produced by FastQC (version 0.11.5)