FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2457929
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9250.035.8026588
GTCTCGC10750.032.1804121
GTCTTGC19250.030.325861
TCGCTCT11950.029.552014
GTATTGC5850.025.8715361
CTGTCGC13150.024.0894079
CTCGCTC12550.023.5450153
TCTCGCT13000.023.2843842
CGCTCTG12850.022.9724045
GAGTGCA56950.022.2507486
GTGTAAA12100.022.038151
GTACTAG5300.021.7571961
GTATAAG6450.021.2301331
GGAGTGC55750.021.050795
GTCTTAC7900.020.9826131
TTGCTCT27300.020.8556274
TTACACG1750.0037883520.5916334
GGCTGGA76700.020.2963161
CTGGAGT65950.020.2169553
CTTGCTC26300.019.7303853
Produced by FastQC (version 0.11.5)