FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2457929
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9700.030.439451
GTCTTGC18800.028.72951
TCGCTCT11200.025.7134654
GTATAAA6500.024.3744051
TTACACT9800.024.2441234
TTAGCGC1500.001556648923.9992353
GAGTGCA59300.023.9182936
GTATTAA6100.023.6115681
GTCTCAC25900.022.8002051
GGAGTGC56000.022.3707165
TCTGTCG11050.022.153148
TTCACCG6950.021.7547025
CTCGCTC12200.021.2452243
TTGCTCT24550.021.1154164
GTATTAG6850.021.0263611
TAGCGCT2404.6065055E-520.9993294
GTTTTAC8300.020.8236961
GGCTGGA76350.020.7509651
GTACTAG3509.3912604E-820.5757941
CCGTGTT7050.020.424889
Produced by FastQC (version 0.11.5)