FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2554087
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCT11300.042.0864834
TCTGTCG10650.039.8853878
GTCTCGC12250.036.469751
CTCGCTC13700.033.661743
CTGTCGC12950.033.3574529
CGCTCTG13750.032.9881365
TCTCGCT13800.032.373512
GTCTTGC24300.031.135811
GTATTAA7200.028.022231
GTACTAA5600.027.0214371
GTACTAT6100.024.8065621
GTGTAGC10600.024.4722441
GTTTCAC39150.023.9270273
GTCGTTC1851.9203353E-423.350228
GTGTAAA15900.022.6594871
GTCCTAT6450.022.3433061
ACCGTGT7750.022.295698
GTATAAA9200.021.930441
GTGTTAA11850.021.8907831
TTGACCG1650.002696165621.8355054
Produced by FastQC (version 0.11.5)