FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2554087
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11900.035.70841
GTCTTGC21700.032.1941761
TCTGTCG9950.030.3917338
TCGCTCT13750.028.2761544
TCTCGCT15600.025.8505132
CTCGCTC15300.024.4704043
GTATTAC5950.024.2090841
CGCTCTG13850.023.913185
TATACTG6100.023.6063825
GGAGTGC54600.023.3404855
GTCCTAC7100.023.3310781
GAGTGCA59800.022.6343946
GGTATAC4850.022.2748531
CTGTCGC14400.021.9998389
GTATAAG6950.021.762051
GTACAGT13500.021.3398571
CTGTTGC27000.021.066519
GTATAGT7200.021.0064241
GTCTCAC30050.020.3722841
GGCTGGA80800.020.233911
Produced by FastQC (version 0.11.5)