FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2437190
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9300.037.1590928
GTCTCGC8800.036.0281751
GTCTTGC21850.031.9884071
TCGCTCT9900.031.2972054
CTGTCGC12100.029.7503079
GTGTAAC6650.027.0888561
GTCCGGT1959.188694E-625.8663831
CGCTCTG10650.025.6886225
GTGTTAG16850.025.6580491
GTGTAGG11600.025.4681931
TCTCGCT11950.024.7222652
CTCGCTC12350.024.5049933
GTTAGCC11900.024.2206233
GATCGTC1801.5949059E-423.998587
GTATTAA7250.023.8531381
GTACTGA8800.022.9270211
GTGTACG2202.3388933E-522.9270211
CTTGCTC28000.022.6462823
GTGTAGA11750.022.076841
TTGCTCT29750.022.0407664
Produced by FastQC (version 0.11.5)