FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2601342
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11100.037.6340751
TCTGTCG11550.033.661838
TCGCTCT12500.031.103534
TCTCGCT13650.027.4329012
CGCTCTG13050.027.0340755
GTCTTGC22450.026.9487841
CTCGCTC13550.026.5678633
GTACGGT1658.831654E-526.180926
TACACCG2051.3625931E-524.5849935
TTACGAC1500.001556521523.9996383
GTACTAT4850.023.7604391
GAGTGCA58500.023.1376696
CTGTCGC16100.022.807119
GGAGTGC53900.022.7083785
GTGTTAG16600.022.5616821
GTGTAAA14500.022.3522241
GTGTTAC9750.022.161181
ACTACGC2954.1472595E-721.965773
GGATCGT3956.4392225E-1021.8730878
TACCGTT1650.002709166821.8174347
Produced by FastQC (version 0.11.5)