FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2481470
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10150.041.849958
GTCTCGC11400.039.19211
TCGCTCT11400.038.5599674
CTGTCGC12550.034.420459
TCTCGCT14000.029.8546282
GTCTTGC21700.028.8915731
CTCGCTC13250.028.8251573
CGCTCTG14050.028.6959555
GTATAGA8800.026.2046891
GTGTTAG16000.023.8708321
TAATACT7500.023.0601254
GTACTCG2852.9493094E-722.7567021
GTCTTAC9900.021.837241
TAATCCG1700.003221781721.1753255
GTATATA10550.021.174881
ATAGCGC2755.4961874E-620.9637533
GTACTAG4902.0008883E-1120.58941
GGATAAG9650.020.1626761
TTGCTCT31200.020.0944614
GTACAGA11850.020.0681461
Produced by FastQC (version 0.11.5)