FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2481470
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8850.034.995381
TCTGTCG9600.033.7496458
GTCTTGC21350.033.398181
GTACGAC900.003754540331.9996643
TCGCTCT10100.027.801694
GGTCCGA1907.535824E-626.5356621
GTCTTAC10900.024.4489781
CTGTCGC12700.023.8107769
TCTCGCT12100.023.805722
TTACACT12750.022.5884
CTTGCTC28900.022.172783
GTATAAC4609.094947E-1221.9207651
GTCCGAC1650.002703060421.8258691
GGATAAC6000.021.6076131
CTCGCTC12400.021.4836443
CCGTGCG2353.915844E-521.4465839
GTATTAA6750.021.340851
CGCTCTG12300.021.072955
TTGCTCT31100.020.8357934
TACACTG14350.020.5712135
Produced by FastQC (version 0.11.5)