FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2947994
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12400.038.314168
GTCTCGC13100.035.694761
GTCTTGC28250.035.1735531
TCGCTCT14100.032.154414
CTCGCTC15600.028.6013323
TCTCGCT16450.028.0183642
CGCTCTG14450.027.3913865
GTTTCAC39350.027.0667513
GTCTCAC29700.026.8142641
TATCCGT1358.445028E-426.6531186
GTATTCG2507.275594E-826.3025781
GGGTTTC42500.026.3025761
GTGTTAG17650.026.079041
TTGCTCT35200.025.351184
CTGTCGC19650.025.2798889
GTCTTAC11050.025.1256371
GTACTAT5250.023.6584031
ACCGTGT8750.023.0349398
CTTGCTC35400.022.9717643
GTATTAG7650.022.9216351
Produced by FastQC (version 0.11.5)