FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17537789
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG37700.021.0686971
GTGTATA40200.018.981889
GTGTTAG46600.018.5943781
GTGTTAA37650.018.4116441
TATACTG30500.017.9388875
GTATTAG21350.017.9252131
GTATTAA24350.016.9029391
GTGTTAC45200.016.7740211
GTCTAAC24050.015.61257651
GTGTAGT54650.015.1947661
ATGTGTA59800.015.1680177
GTGTAGA78450.015.0030991
GTATAAA35650.014.7859641
GTGTAAC38500.014.6291871
GTGTAAT38250.014.5360221
GTATATA20400.014.5124251
GTGTAAA45950.014.4573491
GTACTAA14100.014.33921051
GTATTAC27800.014.2857891
GTGTAGG79100.014.1495151
Produced by FastQC (version 0.11.5)