FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2472726
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9250.037.358738
GTCTCGC10650.036.757141
GTCTTGC20050.036.1562351
TCGCTCT10400.034.6171044
TCTCGCT12400.027.8723532
TTGCTCT29800.027.7866154
GGTATAC4800.027.1849691
CTCGCTC12100.026.778193
CGCTCTG13850.025.9903935
CTGTTGC23950.025.5508889
GTATTAG7800.025.0938191
GTGTTAG15900.024.620351
CTTGCTC28650.024.3781763
TTAGCGC1801.5937383E-424.0011923
CTCGATC8700.023.9977958
CTGTCGC13850.023.9111619
GTCTTAC8850.023.7548481
GTTAGCC11350.023.4725323
GAGTGCA60200.023.4397096
TTCACCG6500.023.2594015
Produced by FastQC (version 0.11.5)