FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2472726
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9700.039.8920631
TCTGTCG9750.031.7485248
GTCTTGC21650.031.6994931
TCGCTCT12850.030.2407264
CGCTCTG13550.029.209565
GTGTAAC6850.028.7776661
TATACGG1050.00799613727.4139925
TCTCGCT14050.027.1605342
GTATTAC4900.026.8200021
CTCGCTC14150.026.4458353
GTATAAC3709.094947E-1225.6521641
GTGTAAG12700.025.2948041
GTCTCAC25050.024.7738531
GGAGTGC56300.024.4133035
GGATAGT6800.023.6208821
TCTGTCA27600.023.4743258
CTGTCGC14800.023.351699
GAGTGCA60450.023.3334486
CGCTATG1550.001888945823.21447
GTAACGC1550.001889167923.2139283
Produced by FastQC (version 0.11.5)