FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3023779
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCT13050.037.5006984
GTCTCGC12750.037.21781
TCTGTCG13150.033.938538
CGCTCTG13650.032.1616335
CTCGCTC16050.030.9401283
TCTCGCT15650.029.9062982
GTCTTGC26200.029.5360281
GTATAGT7450.025.4779591
GTATTAA9250.025.2554871
GTATTGC7400.024.663561
CTGTCGC18850.024.4413099
TATACTG8000.024.2892845
TTGCTCT35100.023.3743044
GTCTTAC10000.023.3613241
GTGCGAA2202.1143434E-523.228591
GTTTCAC39950.022.8788933
GTGTTAC11200.022.8137951
GTACTAC4800.022.8137951
GTGTTAA10700.022.5152971
GAGTGCA72300.022.4966686
Produced by FastQC (version 0.11.5)