FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2671614
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC19900.038.5409361
GTCTCGC10600.036.5221251
TCTGTCG10950.035.5018778
GTACTAT5000.032.139471
GTATTAG8550.031.609791
GTATTAC5500.027.8896221
GTCTCAC27150.027.442041
TCGCTCT13700.026.7941514
CTGTCGC17050.026.1801139
GTATTCG2258.5412285E-725.9712891
TCTCGCT14200.025.8618282
TTCACCG7300.025.6354475
GTATTAA7450.025.4919511
GTTTCAC36450.025.4731463
CGCTCTG13750.025.1263225
CCGTGTT6650.024.9006359
CTCGCTC14500.024.8194663
GTCTTAC8350.024.493881
GTGTTAC10450.024.4645821
GGGTTTC38650.024.3795811
Produced by FastQC (version 0.11.5)