FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2602671
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG657.5988116E-444.3010569
GTCTTGC22800.040.3378941
GTCTCGC10350.036.67251
TCGCTCT12050.035.8321654
CGCTCTG12800.031.4837865
TCTGTCG10550.031.3874558
TCTCGCT14000.028.289092
CTCGCTC14000.028.2716963
TTGCTCT30100.027.7332254
GTATTAG7700.026.5426851
GTGTTAC8850.026.3927271
CTTGCTC31350.025.709743
TAGTACT4550.025.3056494
GTTTCAC32300.025.176373
GTATAAG6200.024.7232231
GGGTTTC35500.024.4678691
GTATTAA6950.024.155751
TCTTGCT34950.024.1058832
TCTGTTG25750.023.4829588
TCTGTCA30200.023.1214778
Produced by FastQC (version 0.11.5)