FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2722990
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10250.047.762138
GTCTCGC11650.046.6541561
TCGCTCT13300.038.983714
TCTCGCT13700.037.319872
GTCTTGC20300.036.4132231
CTGTCGC15800.033.2632339
CTCGCTC14900.032.864343
CGCTCTG14200.030.4201665
GTATTAT7700.027.2936921
GTCTCGA22450.026.9377676
GTATTAG8000.025.3643071
TCGAACT16250.024.8103779
TCTCGAT10050.024.3563127
GTGTTAC9300.024.1564871
GTATAAT7200.024.1564851
GGTCTCG23550.023.8452745
TTGCTCT28600.023.4163514
GTATTAA7250.022.9903131
CTCGATC10700.022.8767228
GTCTCAC28350.022.7505511
Produced by FastQC (version 0.11.5)