FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2722990
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12000.045.0338251
TCTGTCG11350.043.126248
TCGCTCT14050.038.4143834
CTCGCTC15700.033.4604763
GTCTTGC23700.032.35411
TCTCGCT16350.032.1479532
CGCTCTG15100.031.4539995
CTGTCGC16900.030.2479529
GTATTAC4700.026.4143221
GTCTTAC9450.025.501781
GGAGTGC58450.024.1312855
GTCTCAC30000.024.0991821
GTACTGA8800.024.0659891
GTGTTAC8950.023.6626451
TACACCG1851.9264582E-423.3393335
GTATAGC5050.023.137531
GTGTAAG13600.023.089681
GAGTGCA64700.022.6908476
GTGTTAG17750.022.628341
GTATTAG7150.022.47011
Produced by FastQC (version 0.11.5)