FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2841918
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10750.041.8129731
TCTGTCG11650.038.932638
GTCTTGC23950.034.5085831
TCGCTCT12250.032.9198654
TCTCGCT13800.030.7878272
CTGTCGC16450.029.3234929
CTCGCTC14600.028.6075883
GTGTTAG17600.028.0107041
CGCTCTG13500.026.6646185
GTCTTAC10250.025.4628161
GTATTAA7900.024.7778991
TTGCTCT32300.024.3013324
CGTAACC1500.001554884624.0040682
TAATACT7350.023.514194
GTCTCAC27800.023.4706731
CTTGCTC31550.023.0530363
GTGTTAC10500.022.7851811
GGAGTGC62300.022.5343825
GTATAAA9750.022.3071691
GTTTCAC37450.022.3055173
Produced by FastQC (version 0.11.5)