FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2841918
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10850.041.7996528
GTCTCGC12150.036.044621
TCGCTCT12800.033.1739274
GTCTTGC23750.032.8840751
CGCTCTG14100.029.0944795
GTATCGC1255.38218E-428.788223
CTCGCTC14300.028.6875613
CTGTCGC16650.027.6736079
TCTCGCT15200.027.471152
GTATTAA6800.025.7613011
GTCCTAC7700.024.6460931
GTCTTAC10200.024.3301181
GGGTAAT8200.024.033411
GTCTCAC31300.024.019191
GTTTCAC37350.023.123073
GTTCTAC8250.023.0030211
TTGCTCT35000.022.8249474
GTACTAT5500.022.5606561
GGGTTTC41500.022.5126881
CTTGCTC31550.022.3553523
Produced by FastQC (version 0.11.5)