FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2584168
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9700.046.0168278
TCGCTCT12450.039.9157754
GTCTCGC11450.037.3605081
CTGTCGC14900.031.8906089
CTCGCTC13350.031.8299223
TCTCGCT13300.030.8665452
CGCTCTG13500.030.3974955
GTCTTGC20950.028.3789351
GTGTAAC5550.024.8214341
TTGCTCT28450.024.3026584
GTCTTAC8800.023.0696831
GTACTAT4103.8198777E-1122.9893021
GTGTAGC10200.022.7465781
GGAGTGC60150.022.3821965
GTCTTAA7150.022.3091431
GAGTGCA64200.021.8673576
TTAGACT6600.021.824834
GTCTCAC26600.021.5333541
TAATACT6400.021.3815164
GTATAGT5450.021.2857881
Produced by FastQC (version 0.11.5)