FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2584168
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11550.039.2649358
GTCTCGC11300.034.8891751
TCGCTCT12350.033.2177474
GTCTTGC22150.030.9834331
CTGTCGC17750.027.985899
CTCGCTC14700.027.9074273
TCTCGCT14650.027.0326792
GTATTAA5050.026.0229511
CATACCG1400.001043471325.704215
CGCTCTG14200.025.3421785
GTGTTAG13350.025.15661
CTTGTCG2601.226781E-724.9205449
ATACCGT1450.0012802524.8183386
GTTATAA4201.8189894E-1224.3362771
GTATTAC4002.5465852E-1123.727871
GGAGTGC57650.023.5952575
GAGTGCA63500.023.4621246
GTGTAAA12500.023.3628251
TTGCTCT29350.022.3149344
GTACAGA11500.022.220081
Produced by FastQC (version 0.11.5)