FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3062946
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12400.041.803968
GTCTCGC13350.036.172241
CTGTCGC15950.033.853789
GTCTTGC28900.031.42351
TCGCTCT16850.028.6587184
TCTCGCT16600.028.2219582
CGCTCTG15600.027.2291475
CTCGCTC18750.026.1390343
GTACTAC5550.024.6741661
GTTTCAC41250.024.461663
TTGCTCT37400.024.2817964
GTCTCAC32450.024.2099361
GGGTTTC46950.024.1016031
GTGTAAA16950.023.8122371
TAATACT8500.023.74224
GTACAAG10700.023.5757831
TTCACCG8550.022.7354875
ACCGTGT9500.022.7354878
GTATAAT8000.022.523291
GTACTAT6800.022.2583121
Produced by FastQC (version 0.11.5)