FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3062946
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13500.039.9985588
GTCTCGC12050.034.66851
CTGTCGC16400.034.2426729
GTCTTGC25550.034.110541
TCGCTCT14200.031.4354884
CGACTAT1000.0062864328.798966
CTCGCTC15400.028.050943
TTGCTCT35500.027.9877244
GTCTCAC34200.027.1680051
GTGTTAC10650.025.0233941
TCTCGCT16700.025.0071282
GTCTTAC12500.024.777221
CGCTCTG16650.024.2153455
GTATAAG7800.024.0089321
CTTGCTC34400.023.2317223
TCTTGCT40200.022.7473052
TCTGTCA36500.022.6841168
GTATTAG11350.022.2109071
GGAGTGC67550.021.8496955
GTATTAC6600.021.8263031
Produced by FastQC (version 0.11.5)