FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3260018
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11900.045.3759048
GTCTCGC13450.035.371421
CTGTCGC16200.034.2205289
GTCTTGC27000.033.3716051
TCGCTCT14500.031.8157984
TCTCGCT14900.027.091472
TTGCTCT39900.025.653484
CTCGCTC15750.025.1717263
CGCTCTG17100.023.1567485
CTCGATC13550.022.8475768
GTCTCAC32350.022.7277661
GTCTTAC11600.022.3704831
GTTTCAC48050.022.3523793
CTTGCTC36550.022.2854753
GGGTTTC53800.021.8391721
GGAGTGC73600.021.8141385
GAGTGCA81150.021.4702856
GTATTAG11100.021.4299811
TCGATCT13900.021.2363599
TCTTGCT43550.021.0206642
Produced by FastQC (version 0.11.5)