FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3260018
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13950.040.7954941
TCTGTCG15450.039.610468
TCGCTCT15300.034.351914
TCTCGCT16800.032.5759472
CTGTCGC19750.032.4445959
GTCTTGC28450.032.4105951
CTCGCTC17750.030.4216213
CGCTCTG16300.030.035915
GTATTAA8300.027.7735371
GTCTCAC36000.025.2131651
GTACTAC5700.024.0125371
GTATAAA12500.023.0520361
GTCTTAC12550.022.9601961
TTGCTCT39850.022.7646874
GGGTTTC48650.022.5071281
TCTGTCA36000.021.3993578
GGAGTGC75450.020.9934045
TCACTCT41800.020.841484
TCTTGCT43700.020.7626042
GTGTTAG18950.020.5278681
Produced by FastQC (version 0.11.5)