FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3229103
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13150.041.633211
TCTGTCG15650.035.883678
TCGCTCT17500.034.558744
CCGATCG900.003755186231.9988294
CGCTCTG17000.030.0694895
GTACGAC1204.2289143E-429.9989013
CTGTCGC19000.029.9357479
GTCTTGC26500.029.630071
TCTCGCT17700.029.290242
CTCGCTC18600.029.0311993
GTGTACG3351.8189894E-1227.9544281
GCGTAAT1607.158844E-527.001942
CCGTGTT10050.026.5064939
ACCGTGT9250.026.4638988
TTCACCG9650.024.6208615
GTATAAA12650.024.4866851
GTTTCAC46050.024.0772933
GTCTTAC13650.023.7482661
GGGTTTC51550.023.196981
GTGTAAA17950.022.4737551
Produced by FastQC (version 0.11.5)