FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3172187
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12400.047.029428
GTCTTGC28450.041.0384641
GTCTCGC13600.036.5652471
CTGTCGC16250.035.8870669
TCGCTCT15050.034.00014
TTGCTCT35800.031.405084
TCTCGCT16250.030.1587872
CTCGCTC16300.030.0662753
CGCTCTG17150.029.8057825
CTTGCTC35450.028.6656133
GTCTCAC30700.028.6404461
GTCTTAC11550.026.8314911
TCTTGCT43900.025.6105212
TAATACT6500.024.3931374
TCTGTCA37550.023.5831328
GTATAAC4650.023.2485921
GTTAGCC18000.022.8223693
GTGTTAC11800.022.598421
GTTTCAC43150.022.0471023
TCACTCT36350.022.0078124
Produced by FastQC (version 0.11.5)