FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3172187
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14050.042.0193758
GTCTTGC28200.041.886881
GTCTCGC14050.039.9854051
TCGCTCT17100.034.524694
CTCGCTC17750.030.4211043
CTGTCGC19350.030.1381199
TTGCTCT39300.029.4947974
TCTCGCT19000.028.8022772
CGCTCTG17600.028.6350155
TCTGTCA32850.027.8343078
CTTGCTC39200.026.8150633
GTCGTAG2601.2216515E-724.931731
GTCTCAC32750.024.8513721
TCTTGCT46200.024.6253222
TTACACT12700.023.8099024
GTGTTAC10300.023.7752421
GGAGTGC73900.023.6741225
GTCTTAC11350.023.4795131
TACACTG13850.022.8725685
GAGTGCA80400.022.5661016
Produced by FastQC (version 0.11.5)