FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3427999
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12700.040.2497028
CTGTCGC17050.032.0920339
GTCTCGC14300.031.7499851
GTCTTGC29250.031.044431
TCGCTCT15450.029.8531724
TCTCGCT16700.025.8924882
GTCTCAC36500.024.483181
GTCTTAC11900.024.2243461
CTCGCTC16600.023.8777643
ACCGTGT9700.023.0090128
GTATTAA9450.022.8785481
TTGCTCT41800.022.5857244
GGGTTTC51050.022.1637331
GTGTAGA18550.022.1447051
TCTGTCA40600.021.9889098
GTTTCAC47950.021.6427733
GTATAAA13450.021.4326921
GAGTGCA84500.020.9597666
CTTGCTC39900.020.9519333
TTACTCT17650.020.8240154
Produced by FastQC (version 0.11.5)