FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3427999
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC14550.037.1266561
TCTGTCG15300.035.7633868
GTCTTGC28950.034.5822871
TCGCTCT16400.031.1695824
CTGTCGC19200.030.3738759
CGCTCTG17700.028.4735225
GTGTTAG21150.026.2221241
TCTCGCT19850.026.1156652
CTCGCTC18750.025.727053
TACGCCG1701.0819677E-425.4108265
GTCTTAC11800.024.4154931
GTTTCAC46550.024.1280023
GTGTTAC10950.023.6796841
ACCGTGT8600.023.4409968
TTGCTCT38150.023.024054
GGGTTTC51000.022.7375261
GGTATAC5750.022.5471781
GTCTCAC37100.022.5201661
GCGCTAG2954.133217E-721.9739441
GTGTAAC8500.021.184031
Produced by FastQC (version 0.11.5)