FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3144286
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13400.037.6104558
GTCTCGC13300.035.199451
TCGCTCT13050.034.7572484
GTCTTGC26350.034.1667441
CTGTCGC16700.031.0406889
TCTCGCT16350.027.744232
GTATAAG8200.027.2283941
CTCGCTC16600.026.4567853
CGCTCTG15600.026.3066525
GTCTTAC11150.025.8380241
GTCTCAC33300.025.521841
GCGTTAC2101.6379758E-524.0078331
GGTATAC5900.023.1940061
TCTGTCA34900.023.1051038
TTACACT11800.022.5753784
CTTGCTC35350.022.403643
GTCTTAG12600.022.2929861
TACCCCG3305.0571543E-821.8173185
GGAGTGC68200.021.6413755
GAGTGCA73700.021.5893766
Produced by FastQC (version 0.11.5)