FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3027376
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12600.037.7566031
TCTGTCG11100.034.3754588
TCGCTCT13750.031.977664
GTCTTGC25800.031.2908861
CTGTCGC14300.029.703779
TCTCGCT15100.029.1187272
CGCTCTG14600.028.10725
CTCGCTC15500.027.902243
GTCTTAC10700.026.2724381
GTGTTAG21950.025.2857441
GGTATAC6000.025.2282771
TCTCGAT13050.024.8254767
TCGATCT13250.024.4507529
TAGTACG1851.9052849E-423.3775524
GGTTAAC5900.023.2124561
CTCGATC13550.022.8467678
TGTTAGC20150.022.1787052
GGAGTGC67950.022.1438145
GTATAGC6250.021.912561
GTTAGCC17550.021.7679883
Produced by FastQC (version 0.11.5)