FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3027376
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12600.046.8715361
TCTGTCG14750.043.4409038
TCGCTCT14700.042.1193854
CTGTCGC18050.037.8919759
CGCTCTG16350.036.1074645
CTCGCTC16800.035.568843
TCTCGCT17400.033.933612
GTCTTGC26400.031.3732681
GGTATAC5950.029.0509381
GTATTAA8000.027.9085671
TATACGG1306.756685E-427.690295
TTGCTCT34850.026.2362524
GTCTCAC31700.024.3103051
GTGTTAG18900.024.0073721
GTGTAAC7800.023.0840111
GTGTAAG14450.022.9274561
GGCTAGA18350.022.7644821
GGAGTGC68300.022.4522445
GTTTCAC43100.022.216483
GAGTGCA74500.022.0332956
Produced by FastQC (version 0.11.5)