FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2654901
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11800.037.867461
TCTGTCG10000.035.9978378
GTATAAG7500.030.7500041
TCGCTCT13050.030.374474
CTGTCGC14000.030.3410349
TCTCGCT14450.029.9253962
GTCTTGC24450.029.476621
CTCGCTC14450.028.9278813
GTCTTAC10650.027.068671
CGCTCTG14450.025.4102385
GTGTATA13250.025.0206431
ATACGGT2101.642757E-523.9985586
GTCTCAC28800.023.7732011
GTGTTAG16100.023.7250141
GTATAGC6150.023.4375041
GTATTAT9650.022.4052851
GTACGGG2303.2921747E-521.9344481
GTGTAGT15950.021.6888771
TTACACT12650.021.6495864
GAATACG2003.2321896E-421.62111
Produced by FastQC (version 0.11.5)