FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2654901
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11100.040.2133758
GTCTCGC13350.036.6853871
TCGCTCT13450.034.2578054
CGCTCTG14000.032.911965
GTCTTGC23150.029.8665921
CTGTCGC14250.029.3031969
TCTCGCT15700.028.4343242
GTGTAAA17000.027.537841
CTCGCTC15800.026.8841783
TAATACT7250.025.8188654
GTGTTAC10700.022.2124061
GTATTAC6500.022.160631
GTCTAGC11300.021.670351
GTATTAA8450.021.3082981
GTTTCAC39350.021.2234083
TTGCTCT32300.021.1749744
GTCTTAA9900.021.0973661
GGTATAC4801.4551915E-1121.006431
GTGCTAA10000.020.8863931
GTACTGA7600.020.8484861
Produced by FastQC (version 0.11.5)