FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3064498
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12200.041.309238
GTCTTGC27300.034.84371
GTCTCGC13700.033.6645161
CTGTCGC15650.031.7426829
TCGCTCT15600.030.0262974
TCTCGCT16600.026.9151342
CTCGCTC17250.026.7364563
TTGCTCT36650.025.1680154
GTATTAA9600.024.0210341
TATACTT7850.023.8458355
GTACTAA6250.023.0601941
GTACTAT7200.023.0201571
TAATACT8550.022.756774
GTGTAAC8350.022.438811
GTCTCAC33100.021.9890441
GTCCTAT8550.021.9139271
GTATAAA11100.021.4241681
CGCTCTG16200.021.3321745
CTTGCTC37500.021.138513
GTATATA10750.020.7809891
Produced by FastQC (version 0.11.5)