FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2350964
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9650.047.751168
GTCTTGC23300.036.872031
GTCTCGC10750.035.593721
CTGTCGC14350.033.616639
TCGCTCT11150.030.3484024
TTGCTCT30550.029.2228974
TCTCGCT12850.029.1683642
CTTGCTC30750.026.9565683
CTCGCTC13500.026.6961153
GGGTTTC37450.026.603161
GTCTCAC26500.026.4260231
CGCTCTG14000.026.227385
GTTTCAC33700.026.0940693
GTACTCG1959.052632E-625.9160841
TCTGTCA29050.025.7761768
GTATTAG7650.024.5367971
CTGTCAC32150.024.1865589
GTATAAC4800.024.0649361
ACCATGT29150.023.9587658
ATACTGA10650.023.6619076
Produced by FastQC (version 0.11.5)