FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1888969
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6500.048.73838
GTCTTGC17400.039.3978161
CTGTCGC9700.035.628759
GTCTCGC7750.033.5195851
TCGCTCT8450.033.2288974
GTCGAGT1204.2274324E-429.99998
CTATGCG1504.6135043E-528.7999069
GTCCTAC5600.028.3486561
CTCGCTC10000.028.1059463
TTGCTCT24600.027.5106474
GTGTAAC6050.027.4328081
CTTGCTC23700.027.3670373
TCTCGCT10050.027.2490372
GTGTTAC7950.027.230271
GTCTCAC21950.025.3136081
GTACTAT3551.6370905E-1024.3921851
GGTATAC3851.6370905E-1124.3657861
GTACCGG1801.5702285E-424.0534041
TTAGCGC1500.001547880124.0221773
TCTTGCT27950.023.9792022
Produced by FastQC (version 0.11.5)