FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1993792
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9100.040.350058
GTCTTGC19700.039.585681
GTCTCGC8500.034.829361
GTATTAA5400.033.429261
GTATTAC4450.030.8300461
CTGTCGC12150.030.2210279
TCGCTCT9300.030.1923544
GTATAAC3750.028.8828831
GTCTTAC7500.028.8828831
GGGTTTC33300.026.4542941
TTGCTCT26650.026.2053434
TAGCACG2259.6855E-725.5989884
GTATTAG7700.025.3194121
CTTGCTC26950.025.144973
GTGTAAG9550.024.9511831
TCTCGCT10700.024.928722
GTCTAAT5000.024.5504531
GTTTCAC31200.024.4925143
GTATAAT5900.024.4770181
TCTTGCT30400.024.4256272
Produced by FastQC (version 0.11.5)