FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1993792
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10100.044.9142271
TCTGTCG9050.036.596658
GTCTTGC18600.036.3897971
TCGCTCT10850.035.8340644
CTCGCTC12650.031.8734863
TCTCGCT13550.031.3528822
CGCTCTG11100.031.1351015
CTGTCGC11900.029.0419859
CCGTAAC1555.7658875E-527.8730341
GTGTAAA11650.027.8132211
GGATAAC5450.027.7451761
GTGTTAG13550.027.6330491
ACCGCGA1358.419096E-426.6666378
GTATAAG5250.026.0590741
GTGTAAC6650.025.9868891
TTGCTCT26200.025.8320354
GTATTGG6200.025.5502821
CTGTTGC20900.025.4927969
GTCTTAC9650.024.6235891
GTCTAAG7050.024.5124571
Produced by FastQC (version 0.11.5)